|
| |
News
New reagents to chicken co-stimulatory ligands and
receptors
John Young’s group have developed five new monoclonal
antibodies - now made available through AbD Serotec:.
MCA2836 (clone AV79) recognises chicken CD40
MCA2837 (clone IAH:F864:DC7) recognises
chicken CD80
MCA2838 (clone IAH:F853:AG2) recognises
chicken CD86
MCA2839 (clone IAH:F676:BE3) recognises chicken CD275
MCA2840 (clone IAH:F799:IC12) recognises
chicken CD276
 New mAbs to
study bovine NK cells
Shirley Ellis's Group have produced mAbs to
Ly49 and
KIR
New reagents for bovine IL-2
The Morphosys HuCal system has been successfully used
to produce reagents recognizing bovine IL-2 - Jayne Hope's group are testing
these for use in FCM and ELISA
 Reagent
database - 1st phase release due soon
Paul Sopp, Lucy Taylor & John Dale have developed a
reagent database that will be searchable using any web browser and will be made
available to the whole research community. The aim was to compile and
maintain the most complete list of details of reagents for use in veterinary
species. The first phase release will include lists of monoclonal antibodies
and recombinant proteins available for cattle, chickens, horses, pigs and
sheep.
Click here to see screen shots of the database and the data entry
application.
New mAbs to ovine IL-8
Three
monoclonal antibodies to ovine CXCL8/IL-8 developed by the Toolbox
are currently being characterised and evaluated for species cross-reactivity
with cattle and horse IL-8 in conjunction with USDA-VIRN. One monoclonal has
been found to work on fixed sheep tissues
Researchers
benefit from reagent transfers
Gary Entrican and Colin
McInnes have distributed reagents to collaborators and formed domestic and
international links -
see current list.
Reagents for ovine cytokines
Gary Entrican's group
have demonstrated staining of cell lines expressing ovine cytokines
including
Interferon-g,
TNF-a,
IL-8,
IL-4 and
IL-10
 New
reagents to equine molecules
Julia Kydd's group have generated polyclonal antisera (ovine) to equine
TNF-alpha; IL-6; IL-15 and CD14. Recent developments also include production
of CHO-K1 stably transfected with pcDNA3IGHG1 containing
the sequence for RANTES; IL-6; TNF-alpha; Granzyme B
and CD14
Links
DGIL Porcine Immunology
and Nutrition (PIN) database
NIAID, NIH Resources for
Researchers
Equine Immunology
Resource Page
Porcine Immunology
Resources Homepage
Online Resources For
Immunology Research Using Comparative And Veterinary Models
ImMunoGeneTics Database
Comparative
Immunoglobulin Workshop (CIgW)
IPD - MHC Database
Centre For Animal
Functional Genomics
ARK-Genomics
Horse Genome Project
Sheep Genome Resources
NAGRP Cattle Genome
Coordination Program
U.S.
Veterinary Immune Reagent Network
Veterinary Immunology email forum
Recent publications from
The Immunological Toolbox
- Hope, J.C., Kwong, L.S., Thom, M., Sopp, P., Mwangi,
W., Brown, W.C., Palmer, G.H., Wattegedera, S., Entrican, G. and Howard
C.J. (2005). Development of detection methods for ruminant interleukin
(IL)-4. Journal of Immunological Methods 301, 114-123
- Sopp P, Werling D, Baldwin C
Cross-reactivity of mAbs to human CD antigens with
cells from cattle. Vet
Immunol Immunopathol 2007 Jun 20
- Griebel, P.J., Entrican, G., Rocchi, M., Beskorwayne,
T., Davis, W.C., Cross-reactivity of mAbs to human CD antigens with
sheep leukocytes, Vet Immunol Immunopathol 119 (2007)
115-22
- Wattegedera, S. Rocchi, M., Sales, J., Howard, J.C.,
Hope, J.C. and Entrican, G. (2008). Antigen-specific peripheral immune
responses are unaltered during normal pregnancy in sheep. Journal of
Reproductive Immunology 77, 171-178
- Entrican, G., Jepson, C. and Deane, D. Growing hybridomas. Methods
in Molecular Biology. In press.
- Entrican, G., Lunney, J.K, Rutten, V.P. and Baldwin, C.L. A current
perspective on availability of tools, resources and networks for
veterinary immunology. Veterinary Immunology and Immunopathology. In
press.
- Entrican, G., Wattegedera, S., Rocchi, M. and Wheelhouse, N.
Pregnancy, indoleamine 2,3-dioxygenase (IDO) and chlamydial abortion: an
unresolved paradox. Veterinary Microbiology. In press.
-
Entrican, G., Lunney, J.K., Rutten, V.P., Baldwin,C.L., A Current
Perspective on Availability of Tools, Resources, Networks for Veterinary
Immunology, Veterinary Immunology and Immunopathology (2008),
doi:10.1016/j.vetimm.2008.10.291
Posters
|
