News

New reagents for Chicken Cytokines & DC

Pete Kaiser's group have produced a wide range of new reagents for cytokines and DCs (see below)

 

 

Recent Developments

Database / Website

Reagent database - Phase 1 released

Our aim is to compile and maintain the most complete list of reagents for use in veterinary species. The first phase release includes lists of monoclonal antibodies and recombinant proteins available for cattle, chickens, horses, pigs and sheep. The database is currently being populated with reagents.

See full progress report

Chickens

New reagents to chicken co-stimulatory ligands and receptors

John Young’s group have developed five new monoclonal antibodies - now made available through AbD Serotec:

New reagents for Chicken Cytokines & DC

new chicken reagents

See full progress report

Cattle

New mAbs to study bovine NK cells

Shirley Ellis's Group have produced mAbs to Ly49 and KIR

New reagents for bovine IL-2

The Morphosys HuCal system has been tested for the selection of reagents recognizing bovine IL-2 - Jayne Hope's group are comparing these with conventional reagents for detection of IL-2 by FCM and ELISA.

See full progress report

Sheep

New mAbs to ovine IL-8

Three monoclonal antibodies to ovine CXCL8/IL-8 developed by the Toolbox are currently being characterised and evaluated for species cross-reactivity with cattle and horse IL-8 in conjunction with USDA-VIRN. One monoclonal has been found to work on fixed sheep tissues.

Reagents for ovine cytokines

Gary Entrican's group have demonstrated staining of cell lines expressing ovine cytokines including Interferon-g, TNF-a, IL-8, IL-4 and IL-10

Researchers benefit from reagent transfers

Gary Entrican and Colin McInnes have distributed reagents to collaborators and formed domestic and international links - see current list.

See full progress report

Horses

New reagents to equine molecules

Julia Kydd's group have generated polyclonal antisera (ovine) to equine TNF-alpha; IL-6; IL-15 and CD14. Recent developments also include production of CHO-K1 stably transfected with pcDNA3IGHG1 containing the sequence for RANTES; IL-6; TNF-alpha; Granzyme B and CD14

See full progress report

Contact Information

The consortium leader is Dr Gary Entrican from the Moredun Research Institute, Edinburgh

Telephone +44-131-445 5111
Fax +44-131-445 6235
Postal Address Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, EH26 0PZ
Email General Information: gary.entrican@moredun.ac.uk
Website Information: toolbox@bbsrc.ac.uk

Send mail to toolbox@bbsrc.ac.uk with questions or comments about this web site.