PPT23

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MOUSE ANTI-PORCINE CD8.4b

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Flow Cytometry [FC]
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Immunohistochemistry- Frozen [IHCF]
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Immunohistochemistry- Paraffin [IHCP]
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Immunocytochemistry [IHC]
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Immunoprecipitation [IP]
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ELISA [E]
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Western Blotting [WB]
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Cell function [CF]
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Affinity sorting [AS]
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In vivo depletion [IVD]
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ELISPOT [ES]
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Documents

Name Description
CD8beta versus CD27.pdf

Comments

Monoclonal antibody PPT23 recognizes the porcine CD8-beta chain and is well suited for flow cytometry. It can be used to identify conventional CD8 T cell in the pig, which have the capacity to differentiate into cytotoxic T cells. Example shows co-staining with CD27 - CD27 aides in the identification of early effector and naive CD8 T cells. In this example the PPT23 mAb was biotinylated and fluorescence-labelled with Streptavidin-BV421. The mAb used for CD27 staining (clone b30c7) was conjugated to AlexaFluor647.

References

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