VIV-KM1

back

Supplier:
Mouse anti-porcine CD335

Please submit additional references, images or any other useful information through the comments section.

Flow Cytometry [FC]
0
Immunohistochemistry- Frozen [IHCF]
0
Immunohistochemistry- Paraffin [IHCP]
0
Immunocytochemistry [IHC]
0
Immunoprecipitation [IP]
0
ELISA [E]
0
Western Blotting [WB]
0
Cell function [CF]
0
Affinity sorting [AS]
0
In vivo depletion [IVD]
0
ELISPOT [ES]
0
Virus neutralization [VN]
0
Hemagglutination inhibition [HAI]
0
Log in or register to rate applications

Documents

Name Description
NKp46 versus CD8alpha.pdf NKp46 expression in combination with CD8alpha on blood-derived porcine lymphocytes

Comments

Monoclonal antibody VIV-KM1 recognizes porcine NKp46 (CD335, doi: 10.1002/eji.201141989). Different from several other species, NKp46 is not expressed on all porcine NK cells but NKp46 expressing porcine NK cells seem to be in a less differentiated stage than their NKp46-negative counterparts (doi: 10.1186/1297-9716-44-13). NKp46 is also expressed on a small subset of porcine T cells, which have NK-cell characteristics (doi: 10.3389/fimmu.2016.00263). The staining example shows NKp46 expression in combination with CD8alpha on blood-derived porcine lymphocytes. Anti-NKp46 mAb VIV-KM1 was conjugated to AlexaFluor647, CD8alpha was labelled with mAb 11/295/33. Monoclonal antibody 11/295/33 was labelled with an isotype-specific secondary antibody conjugated with PE-Cy7 (goat-anti mouse IgG2a-PE-Cy7).

References

Mair KH, Müllebner A, Essler SE, Duvigneau JC, Storset AK, Saalmüller A, Gerner W.: Porcine CD8αdim/-NKp46high NK cells are in a highly activated state. Vet Res, 2013; 44:13

link
Kerstin H. Mair, Sabine E. Essler, Martina Patzl, Anne K. Storset, Armin Saalmüller, Wilhelm Gerner: NKp46 expression discriminates porcine NK cells with different functional properties European Journal of Immunology, 44289; 42:1261-1271 link
Kerstin H. Mair, Maria Stadler, Stephanie C. Talker, Hilde Forberg, Anne K. Storset, Andrea Müllebner, J. Catharina Duvigneau, Sabine E. Hammer, Armin Saalmüller, Wilhelm Gerner: Porcine CD3+NKp46+ Lymphocytes Have NK-Cell Characteristics and Are Present in Increased Frequencies in the Lungs of Influenza-Infected Animals Frontiers in Immunology, 48504; 7: link
Kerstin H Mair, Andrea Müllebner, Sabine E Essler, J Catharina Duvigneau, Anne K Storset, Armin Saalmüller, Wilhelm Gerner: Porcine CD8αdim/-NKp46high NK cells are in a highly activated state Veterinary Research, 45136; 44:13 link